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Scope advance reveals first look through all cortical layers of awake brain

Three-dimensional rendering of a sequence of 450 lateral three-photon images acquired with 2-?m increment from the visual cortex (layer 1 on the left to the subplate on the right). Green color represents GCaMP6s signal, and magenta color represents label free THG signal generated in the blood vessels and myelin fibers in the white matter. Scale bar, 100 ?m.

Credit: Murat Yildirim et. al.

Three-dimensional rendering of a sequence of 450 lateral three-photon images acquired with 2-?m increment from the visual cortex (layer 1 on the left to the subplate on the right). Green color represents GCaMP6s signal, and magenta color represents label free THG signal generated in the blood vessels and myelin fibers in the white matter. Scale bar, 100 ?m.
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