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Humble dung beetles may be ideal DNA detectors for animal surveys

Humble dung beetles may be ideal DNA detectors for animal surveys
Science
Biologists wanting to identify the resident creatures of a location, be it nearby woods or a distant mountainside, could soon have new assistants to help them vacuum up a surroundings DNA: dung beetles. The guts of these unfussy eaters contain species-identifying mammalian DNA, a study has found, and could provide an easy, low-tech way tocatalogregional biodiversity.

Using insects to sample biodiversity isa super idea,says Elizabeth Hadly, an environmental biologist at Stanford University who was not involved in the study.Dung beetles are everywhere.

Attempts to figure out a regions biodiversity through environmental DNA (eDNA) samples are only a few decades old. Scientists sift through dirt, soil, and especially water to find loose skin flakes, mucus, and other body fluids; then, they screen these for traces of identifiable DNA to determine which animals live in the region. So far, marine science has been the biggest beneficiary of eDNA techniques, also known as DNA barcoding. DNA can persist in water for several days, allowing scientists to simply filter samples from, say, a pond to get a rough estimate of which species are present.

Its much easier to filter large amounts of water for DNA than it is dirt or soil, so the use of eDNA on solid ground is rarer. Some scientists have scoured the blood in the guts of mosquitoes and leeches to find the DNA of their hosts. But leeches range is limited, and in certain situationslike when lots of humans are aroundmosquitoes can be hard to trap.

Rosie Drinkwater, a molecular biologist and postdoc at Queen Mary University of London, decided to test another widespread connoisseur of DNA-rich body material:the dung beetle. These invertebrates, which comprise thousands of different species within the familyScarabaeidae, feed on other animals feces. There are dung beetles on every continent except Antarctica, and they make their homes in environments from deserts to forests to oil palm plantations, Drinkwater says.

She and colleagues set up baited traps to catch dung beetles in a forest on Borneo, where Drinkwater has been studying leeches for several years. Their lure? The most reliable source of dung is from your fellow humans, Drinkwater says. All in the name of science. Over 24 hours, the researchers trapped 24 thumb-size dung beetles of the genusCatharsius. Researchers dissected the beetles at a sterile field site and sent their guts to be analyzed at a laboratory at Queen Mary.

After sequencing the DNA inside the guts, they compared it with the genomes of animals known to live in the Bornean forests. DNA sequences solidly matched the bearded pig, sambar deer, muntjac (a type of deer), mousedeer, and porcupine,, the researchers reported on 10 February in a paper posted to the bioRxiv preprint server. They also detected DNA from a rarer banded civet, but the sequences werent clear enough to confidently identify it. Finally, they detected plenty of human DNA not belonging to the provider of the bait; most of it likely came from people working in logging and palm oil harvesting operations nearby. Taken together, the results suggest the technique could indeed be used in eDNA screening elsewhere.

Dung passes through dung beetles relatively quickly, within about 48 hours. Although thats a short window of opportunity, Drinkwater explains, it has an upside: If you get a dung beetlegut DNA hit on a species, you can be relatively certain that animal was nearby recently.

Drinkwater cautions that the study has not yet undergone peer review, and more work is needed to test the strategy in different environments and using different beetles. Still, Dung beetles are a smart addition to the arsenal of eDNA tools, says Michael Kinnison, an evolutionary biologist and ecologist at the University of Maine, Orono.

Hadly agrees, but shes less enthusiastic about the unpleasant fate met by these six-legged citizen scientists. Sacrificing a lot of dung beetles to subsample their guts seems unnecessary, she says, adding she would prefer greater investment in nonlethal techniques such as sampling soils and sediments. However, for particular questions this might be an opportune approach.
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